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Cells were harvested from the filtered liquid by centrifugation at 15,000?g for 10 min at 5°C, washed three times in sterile 0.01 N H2SO4 at pH 2.1. Washed cells were pelleted again by centrifugation, frozen, and stored at ?20°C until protein isolation.
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Australian Capital Territory: Bonython ACT, O'Malley ACT, Campbell ACT, Molonglo ACT, Turner ACT, ACT Australia 2658
New South Wales: Wooloweyah NSW, Sutton Forest NSW, Glenbrook NSW, Congo NSW, Hargraves NSW, NSW Australia 2052
Northern Territory: Katherine NT, Timber Creek NT, Haasts Bluff NT, Yuendumu NT, Milikapiti NT, Stapleton NT, NT Australia 0868
Queensland: Sapphire QLD, Dunwich QLD, Wyreema QLD, Ravenswood QLD, QLD Australia 4015
South Australia: Houghton SA, Hallett SA, Pygery SA, Tumby Bay SA, North Beach SA, Coorong SA, SA Australia 5025
Tasmania: Rowella TAS, Oakdowns TAS, Quoiba TAS, TAS Australia 7036
Victoria: Woodford VIC, Springfield (Macedon Ranges) VIC, Warrong VIC, Cobaw VIC, Yarram VIC, VIC Australia 3007
Western Australia: Pia Wadjari Community WA, Ngalingkadji Community WA, Youndegin WA, WA Australia 6099
British Columbia: Comox BC, Port Coquitlam BC, Kaslo BC, Midway BC, Nakusp BC, BC Canada, V8W 2W4
Yukon: Haines Junction YT, Clinton Creek YT, Whitefish Station YT, Stony Creek Camp YT, Rancheria YT, YT Canada, Y1A 6C9
Alberta: Whitecourt AB, Milk River AB, Tofield AB, Oyen AB, Red Deer AB, Turner Valley AB, AB Canada, T5K 4J5
Northwest Territories: Salt Plains 195 NT, Sachs Harbour NT, Sambaa K'e NT, Yellowknife NT, NT Canada, X1A 5L5
Saskatchewan: Dundurn SK, Arran SK, Punnichy SK, Dorintosh SK, Creelman SK, Parkside SK, SK Canada, S4P 2C3
Manitoba: Gilbert Plains MB, Powerview-Pine Falls MB, Neepawa MB, MB Canada, R3B 2P4
Quebec: Prevost QC, L'Ile-Cadieux QC, Dunham QC, Sherbrooke QC, Saint-Raymond QC, QC Canada, H2Y 3W8
New Brunswick: Petitcodiac NB, Petit-Rocher NB, Paquetville NB, NB Canada, E3B 2H6
Nova Scotia: Colchester NS, Stewiacke NS, West Hants NS, NS Canada, B3J 6S3
Prince Edward Island: Miscouche PE, Tyne Valley PE, Stratford PE, PE Canada, C1A 9N3
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